Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer

CN Johnstone; YE Smith; Y Cao; AD Burrows; RSN Cross; X Ling; RP Redvers; JP Doherty; BL Eckhardt; AL Natoli; CM Restall; E Lucas; HB Pearson; S Deb; KL Britt; A Rizzitelli; J Li; JH Harmey; N Pouliot; RL Anderson

Journal article

Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer

CN Johnstone, YE Smith, Y Cao, AD Burrows, RSN Cross, X Ling, RP Redvers, JP Doherty, BL Eckhardt, AL Natoli, CM Restall, E Lucas, HB Pearson, S Deb, KL Britt, A Rizzitelli, J Li, JH Harmey, N Pouliot, RL Anderson

Dmm Disease Models and Mechanisms | Published : 2015

DOI: 10.1242/dmm.017830

Abstract

The translation of basic research into improved therapies for breast cancer patients requires relevant preclinical models that incorporate spontaneous metastasis. We have completed a functional and molecular characterisation of a new isogenic C57BL/6 mouse model of breast cancer metastasis, comparing and contrasting it with the established BALB/c 4T1 model. Metastatic EO771.LMB tumours were derived from poorly metastatic parental EO771 mammary tumours. Functional differences were evaluated using both in vitro assays and spontaneous metastasis assays in mice. Results were compared to non-metastatic 67NR and metastatic 4T1.2 tumours of the 4T1 model. Protein and transcript levels of markers of..

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University of Melbourne Researchers

Ryan Cross Author

Kara Britt Author

Jason Li Author

Robin Anderson Author

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Grants

Awarded by National Breast Cancer Foundation

Funding Acknowledgements

This work was supported in part by a Career Fellowship from the National Breast Cancer Foundation (NBCF), Australia (to R.L.A.), and project grants from the National Health & Medical Research Council (#566871 and #1020280 to R.L.A. and C.N.J., and #509131 to N.P.). K.L.B. is supported by a National Breast Cancer Foundation Early Career Fellowship. This work was also supported in part by The Higher Education Authority of Ireland PRTLI Cycle 4, National Biophotonics and Imaging Platform of Ireland (Y.E.S. and J.H.H.).